Thalassiosira pseudonana is a marine diatom, a unicellular photosynthetic alga. Data Release Policy As a public service, the raw sequence data from the Thalassiosira pseudonana genome sequencing project is … We report the 34 million–base pair draft nuclear genome of the marine diatom Thalassiosira pseudonana and its 129 thousand–base pair plastid and 44 thousand–base pair mitochondrial genomes. These include genome sequences for a few free-living marine protists such as the red algae Cyanidioschyzon merolae, the diatom Thalassiosira pseudonana, the prasinophytes O. tauri and Ostreococcus lucimarinus, the green algae Chlamydomonas reinhardtii, and the ciliates Paramecium tetraurelia and Tetrahymena thermophila. The marine centric diatom Thalassiosira pseudonana was chosen as the first eukaryotic marine phytoplankton for whole genome sequencing because this species has served as a model for diatom physiology studies, the genus Thalassiosira is cosmopolitan throughout the world's oceans, and the genome is relatively small at 34 mega base pairs. Here, we describe the first system for genetic transformation of Thalassiosira pseudonana (Hustedt) Hasle et Heimdal, the only diatom for which a complete genome sequence is presently available. Specifically, after approximately 60 generations of propagation in E. coli, 17% of cloned T. pseudonana mitochondrial genomes contained deletions compared to 0% of previously cloned P. tricornutum mitochondrial genomes. PLEASE NOTE: The Thalassiosira pseudonana genome sequence is composed of "finished chromosomes" (Thaps3) and "unmapped sequence" (Thaps3_bd), which were annotated separately. They do so, in theory, by sensing changing conditions and adapting their physiology accordingly. Please use both portals to make a complete analysis of the genome. In contrast to higher plants, diatoms acquired their plastids via secondary endosymbiosis, the merging of a eukaryotic host cell with a eukaryotic unicellular photosynthetic alga. P. tricornutum , however, is an atypical diatom in that it does not have an obligate requirement of silicon for growth and exists as three distinct morphotypes: oval, … Diatoms are unicellular algae with plastids acquired by secondary endosymbiosis. This genome instability is potentially due to the lower G+C DNA content of T. pseudonana (30%) compared to P. tricornutum (35%). Marine diatoms are important primary producers that thrive in diverse and dynamic environments. They are responsible for ∼20% of global carbon fixation. A hallmark of diatoms is their intricately patterned silicified cell wall. This method is based on microparticle bombardment followed by selection of transformants using the antibiotic nourseothricin. Genome Insider Episode 7: Decoding Yellowstone’s microbial mats In Hot Spring Microbial Mat, Viruses Ride “Piggyback” The More the Merrier: Making the Case for Plant Pan-genomes Effects of Polar Light Cycle on Microbial Food Web Genome Insider (Mini-)Episode … We report the 34 million–base pair draft nuclear genome of the marine diatom Thalassiosira pseudonana and its 129 thousand–base pair plastid and 44 thousand–base pair mitochondrial genomes. The genome of the diatom Thalassiosira pseudonana: ecology, evolution, and metabolism. T. pseudonana was selected for this study because it is a model for diatom physiology studies, belongs to a genus widely distributed throughout the world's oceans, and has a relatively small genome at 34 mega base pairs. Using the model species Thalassiosira pseudonana , we conducted a detailed physiological and transcriptomic survey to measure the recurrent transcriptional changes that characterize typical … Thalassiosira pseudonana is a species of marine centric diatoms.It was chosen as the first eukaryotic marine phytoplankton for whole genome sequencing. Genome analysis reveals that T. pseudonana contains three silicon transporters and three silaffin genes . To better understand the interplay between redox balance and organic matter secretion, we reconstructed a genome-scale metabolic model of Thalassiosira pseudonana strain CCMP 1335, a model for diatom molecular biology and physiology, with a 60-year history of studies.